Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: List of primary antibodies

Article Snippet: KIF5A , Total Extracts DRG , 1:1.000 , Proteintech (21186-1-AP).

Techniques: Isolation

Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: Effect of short-term diabetes on KIF5A. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of KIF5A. b KIF5A protein levels analyzed by immunoblotting in DRG total extracts of Ctrl and STZ M and F rats. c KIF5A protein levels analyzed by immunoblotting in isolated axoplasm of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05; ### p < 0.001) followed by Bonferroni post hoc test (significance: * p < 0.05)

Article Snippet: KIF5A , Total Extracts DRG , 1:1.000 , Proteintech (21186-1-AP).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Isolation

Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: List of primary antibodies

Article Snippet: DRP1 , Total Extracts DRG , 1:500 , Cell signaling (14647S).

Techniques: Isolation

Journal: Biology of Sex Differences

Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

doi: 10.1186/s13293-018-0164-z

Figure Lengend Snippet: Effect of short-term diabetes on proteins involved in mitochondrial fission/fusion: MFN2, Drp1 and OPA1. a Immunoblot of DRP1 phosphorylation from DRG total extracts of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. The bar graph represents densitometric analysis and statistical evaluation of DRP1 phosphorylation over total DRP1 (DRP1P/DRP1 tot); representative western blot of phosphorylated and total DRP1 is presented above the graph. b Immunoblot of MFN2 from DRG total extracts of Ctrl and STZ M and F rats. c Immunoblot of OPA1 from DRG total extracts of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05) followed by Bonferroni post hoc test (significance: * p < 0.05)

Article Snippet: DRP1 , Total Extracts DRG , 1:500 , Cell signaling (14647S).

Techniques: Western Blot